https://www.isj.unimore.it/index.php/ISJ/issue/feed Invertebrate Survival Journal 2024-02-23T09:12:25+00:00 Prof. Davide Malagoli isj@unimore.it Open Journal Systems <p>An international and open access journal published by Univeristy of Modena and Reggio Emilia devoted to prompt and innovative studies on the basic defense mechanisms in invertebrates</p> https://www.isj.unimore.it/index.php/ISJ/article/view/815 Sequence characterization and expression analysis of a PDGF/VEGF related factor gene in swimming crab Portunus trituberculatus 2024-02-23T09:12:25+00:00 Y Gu none@isj.unimore.it Z Qu none@isj.unimore.it J-J Hu none@isj.unimore.it M-Q Wang wangmengqiang@ouc.edu.cn <p class="p1">&nbsp;</p> <p class="p2">PDGF/VEGF factors are closely associated with immune defense systems. In this study, a PDGF/VEGF related factor gene (designated as PtPVF) from swimming crab <em>Portunus trituberculatus </em>was identified and analyzed. Its full-length cDNA is 1320 bp, containing an open reading frame (ORF) of 582 bp, 5’ untranslated regions (UTR) of 105 bp and 3’ UTR of 633 bp, which encoded a polypeptide of 193 amino acid residues. The deduced amino acid sequence of PtPVF contained a signal peptide, a typical PDGF/VEGF domain, and a cysteine knot motif (CXCXC). Quantitative real-time PCR analysis demonstrated that the mRNA of PtPVF was detectable in gills, gonads, heart, hemocytes, hepatopancreas, intestines, muscles, optic stalk, stomach, and the highest level was found in hemocytes, followed by gill and hepatopancreas. After stimulation by <em>Vibrio alginolyticus</em>, pentachlorophenol (PCP) or cadmium chloride (CdCl<span class="s2">2</span>), the relative mRNA expression levels of PtPVF in hemocytes reached their respective peaks at 3 h, 6 h and 6 h, and 36.61-fold, 3.06-fold and 21.26-fold compared with the original level, respectively, and the respond to <em>V. alginolyticus </em>and CdCl<span class="s2">2 </span>is earlier than to PCP, while under the challenge of <em>V. alginolyticus </em>and CdCl<span class="s2">2</span>, the mRNA expression level of PtPVF was significantly higher than that of PCP stimulation. Furthermore, the mRNA expression level of PtPVF first decreased at 3 h and then increased rapidly to a peak at 6 h after PCP challenge. All these results demonstrated that PtPVF might be involved in the response to invading pathogens and environmental pollution stimulants of <em>P. trituberculatus</em>.</p> 2024-02-23T00:00:00+00:00 Copyright (c) 2024 Y Gu, Z Qu, J-J Hu, M-Q Wang