Effect of andrographolide on phosphatases activity and cytotoxicity against Spodoptera litura

Authors

  • E Edwin Division of Biopesticides and Environmental Toxicology, Sri Paramakalyani Centre for Excellence in Environmental Sciences, Manonmaniam Sundaranar University, Alwarkurichi - 627 412, Tirunelveli, Tamil Nadu, India
  • P Vasantha-Srinivasan Division of Biopesticides and Environmental Toxicology, Sri Paramakalyani Centre for Excellence in Environmental Sciences, Manonmaniam Sundaranar University, Alwarkurichi - 627 412, Tirunelveli, Tamil Nadu, India
  • S Senthil-Nathan Division of Biopesticides and Environmental Toxicology, Sri Paramakalyani Centre for Excellence in Environmental Sciences, Manonmaniam Sundaranar University, Alwarkurichi - 627 412, Tirunelveli, Tamil Nadu, India
  • A Thanigaivel Division of Biopesticides and Environmental Toxicology, Sri Paramakalyani Centre for Excellence in Environmental Sciences, Manonmaniam Sundaranar University, Alwarkurichi - 627 412, Tirunelveli, Tamil Nadu, India
  • A Ponsankar Division of Biopesticides and Environmental Toxicology, Sri Paramakalyani Centre for Excellence in Environmental Sciences, Manonmaniam Sundaranar University, Alwarkurichi - 627 412, Tirunelveli, Tamil Nadu, India
  • S Selin-Rani Division of Biopesticides and Environmental Toxicology, Sri Paramakalyani Centre for Excellence in Environmental Sciences, Manonmaniam Sundaranar University, Alwarkurichi - 627 412, Tirunelveli, Tamil Nadu, India
  • K Kalaivani Post Graduate and Research Department of Zoology, Sri Parasakthi College for Women,Courtrallam-627 802, Tirunelveli, Tamil Nadu, India
  • W B Hunter United States Department of Agriculture, ARS, U.S. Horticultural Research Laboratory, 2001 South Rock Road, Fort Pierce, FL 34945, USA
  • V Duraipandiyan Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University, P.O.Box.2455, Riyadh 11451, Kingdom of Saudi Arabia
  • N A Al-Dhabi Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University, P.O.Box.2455, Riyadh 11451, Kingdom of Saudi Arabia

DOI:

https://doi.org/10.25431/1824-307X/isj.v13i1.153-163

Keywords:

biorational insecticides, HPLC, GC-Mass, toxicity, Noctuidae, histology

Abstract

Andrographolide was isolated from ethanol extraction of Andrographis paniculata by column
chromatography. Evaluation of larvicidal efficacy, enzymatic changes and cytotoxic activities against Spodoptera litura were conducted across a range of concentrations. The compound showed significant larvicidal activity between 5 - 25 ppm, post ingestion. Morphological deformities observed in larval-pupal stages. Enzymatic profiles were altered by reduction in acid phosphatase, ACP activity by 69.18 %, alkaline phosphatase, ALP activity 75.3 % and 74.9 % reduction in ATPase. Binding affinity to midgut epithelium cells suggests disintegration of cellular organelles observed was directly associated with ingestion of the compound. The results suggest that andrographolide has potential for development as a significant inhibitor of development against the pest Spodoptera litura.

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Published

2016-05-12

Issue

Vol. 13 No. 1 (2016)

Section

Research Reports