Application of MALDI-MSI for detection of antimicrobial peptides in tissues of the marine invertebrate Arenicola marina

Authors

  • A L Maltseva Department of Invertebrate Zoology, St Petersburg State University, St Petersburg, Russia
  • V V Starunov Department of Invertebrate Zoology, St Petersburg State University, St Petersburg, Russia
  • P A Zykin Department of Cytology and Histology, St Petersburg State University, St Petersburg, Russia

DOI:

https://doi.org/10.25431/1824-307X/isj.v13i1.205-209

Keywords:

MALDI-MSI, antimicrobial peptides, polychete immunity, Arenicola marina

Abstract

MALDI imaging mass-spectrometry (MALDI-MSI) is a highly informative approach combining
morphology with molecular data. It is widely applied in neuroscience, plant science, cancer-biology, biomedicine, including clinical, and preclinical studies, but not for investigation of endogenous peptides/proteins or metabolites in marine invertebrates. We examined the informativeness of MALDIMSI for analysis of distribution of antimicrobial peptides (arenicins) in the polychete Arenicola marina and concluded that it can be successfully used as a primary rough express screening method.

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Published

2016-06-13

Issue

Vol. 13 No. 1 (2016)

Section

Short Communications