Effects of fluoride on primary cultured haemocytes from the marine gastropod Haliotis tuberculata

Authors

  • R Ladhar-Chaabouni Marine Ecotoxicology UR 09-03, IPEIS BP 805, 3018 Sfax, Tunisia
  • T Houel Normandie université F-14032 Caen, France ; UMR BOREA (Biologie des Organismes et des Ecosystèmes Aquatiques), MNHN, UPMC, UCBN, CNRS-7208, IRD-207, IBFA, Université de Caen Normandie, Esplanade de la Paix, F-14032 Caen Cedex 5, France
  • J M Lebel Normandie université F-14032 Caen, France ; UMR BOREA (Biologie des Organismes et des Ecosystèmes Aquatiques), MNHN, UPMC, UCBN, CNRS-7208, IRD-207, IBFA, Université de Caen Normandie, Esplanade de la Paix, F-14032 Caen Cedex 5, France
  • A Hamza-Chaffai Marine Ecotoxicology UR 09-03, IPEIS BP 805, 3018 Sfax, Tunisia
  • A Serpentini Normandie université F-14032 Caen, France ; UMR BOREA (Biologie des Organismes et des Ecosystèmes Aquatiques), MNHN, UPMC, UCBN, CNRS-7208, IRD-207, IBFA, Université de Caen Normandie, Esplanade de la Paix, F-14032 Caen Cedex 5, France

DOI:

https://doi.org/10.25431/1824-307X/isj.v0i0.1-7

Keywords:

Fluoride, Haemocytes, Haliotis tuberculata, Immune parameters, In vitro, Primary culture

Abstract

As a consequence of human’s activities, fluoride concentration in many aquatic ecosystems is significantly increasing. Nevertheless, little is known about fluoride toxicity to aquatic life. In this study the effect of exposure to different concentrations of sodium fluoride (2, 10, 50, 250 and 1,250 μg mL−1) during 24 h on primary cultured haemocytes of the gastropod Haliotis tuberculata was realized. Results indicate no significant effect of NaF on cell viability, Lysosomal membrane stability, phagocytosis and ROS production at concentrations of 2, 10, 50 and 250 μg mL−1. Nevertheless, lysosomal membrane alterations, a decrease of phagocytosis and morphological changes of H. tuberculata haemocytes were observed at concentration of 1,250 μg mL−1 NaF suggesting a potential impact of NaF at high concentration in the environment.

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Published

2019-01-03

Issue

Section

Research Reports