First evidence of antimicrobial activity of neurotoxin 2 from Anemonia sulcata (Cnidaria)
Keywords:antimicrobial peptide, Anemonia sulcata, ATX II, neurotoxin, Micrococcus lysodeikticus
We investigated the antibacterial activity of Anemonia sulcata (Cnidaria, Anthozoa) tentacle and
body acidic extracts. Biochemical purification consisted of first step on solid phase Sep-Pak C8 column followed by several HPLC runs on C18 column using different conditions. Anti-Micrococcus lysodeikticus activity has been detected in 40 % acetonitrile fractions. The resulting purified molecule from tentacles had a molecular mass determined by MALDI-TOF mass spectrum of 4946,299 Da and has been completely sequenced. Its aa sequence revealed identity with the Neurotoxin 2 (ATX-II), a Na+ channel blocking toxins. Consequently, ATX-II appeared to display a dual role as toxin and as antibacterial.