Composition and diversity analysis of intestinal microbiota in the fifth instar silkworm, Bombyx mori L.

Authors

  • C Hou Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang Jiangsu 212018, China ; The Key Laboratory of Silkworm and Mulberry Genetic Improvement, Ministry of Agriculture, Sericultural Research Institute, Chinese Academy of Agricultural Science, Zhenjiang Jiangsu 212018, China
  • Y Shi Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang Jiangsu 212018, China
  • H Wang Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang Jiangsu 212018, China
  • R Li Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang Jiangsu 212018, China
  • M A Nartey Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang Jiangsu 212018, China
  • X Guo Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang Jiangsu 212018, China; The Key Laboratory of Silkworm and Mulberry Genetic Improvement, Ministry of Agriculture, Sericultural Research Institute, Chinese Academy of Agricultural Science, Zhenjiang Jiangsu 212018, China

DOI:

https://doi.org/10.25431/1824-307X/isj.v15i1.223-233

Keywords:

Silkworm, Gut microbiota, Composition, Pyrosequencing 16S rRNA genes

Abstract

Despite the low diversity and high variability at intra- and interspecific levels, gut microbiota of insect play important roles in digesting and absorbing nutrients, health and development, even in resistance and immunity. In this study, we investigate the composition and diversity of gut microbiota in the 5 th instar of silkworm larvae since the mount of ingested mulberry leaves of this stage is about 85% of the total amount. Intestinal contents were collected at 0, 12, 24, 48 and 72h in the 5 th instar. 32, 21, 29, 24, 14 phyla and 386, 163, 292, 196, 144 genera were detected respectively in the samples described above. In general, the dominated phyla in the larvae gut were Firmicutes, Cyanobacteria, Proteobacteria, Actinobacteria and Bacteroidetes; the dominated genera were Enterococcus, Staphylococcus, Arthrobacter, Pseudomonas, Lactobacillus, Bacteroides, Paenibacillus and Serratia, respectively. The range of phyla and genera was different and there were two peaks of microbial diversity at 0 and 24 h. Compared with the intestinal bacteria of these time points, there were unique bacteria and 56 common ones. In these bacteria, the abundance of Gram-positive bacteria including Enterococcus and Staphylococcus were the highest. Our results provided foundation and reference on the research of intestinal microorganisms and resistance breeding in silkworm.

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Published

2018-06-12

Issue

Section

Research Reports