Differentially expressed genes in the midguts of BmNPV-susceptible and resistant silkworm strains determined using suppression subtractive hybridization
Keywords:Bombyx mori, nuclear polyhedrosis virus, suppression subtractive hybridization, antivirus
Bombyx mori nuclear polyhedrosis virus (BmNPV) has caused great economic losses to sericulture and considerable effort has been made to identify disease resistance genes in B. mori. We constructed differential expression gene libraries of the resistant near-isogenic line (NIL) BC10 and susceptible strain 306 using the suppression subtractive hybridization technique. A total of 23 differentially expressed genes were obtained, of which 17 genes were upregulated in BC10, and 6 genes were upregulated in 306. These differentially expressed genes are involved in cell metabolism, transmembrane transport, cytoskeleton, protease, development and immunity. Six BC10 upregulated genes were verified by real-time quantitative PCR, and the results were consistent with those of subtractive hybridization. The resistance trait of NIL BC10 was inherited from the resistant strain NB, and its genetic background was 99.99% similar to that of recurrent parent 306. The upregulated genes in NIL BC10 may be associated with silkworm BmNPV resistance.