Oxidative stability of the hemolymph in different crustacean species

Authors

  • B Beer Ljubić Internal Diseases Clinic, Faculty of Veterinary Medicine University of Zagreb, Heinzelova 55, 10000 Zagreb, Croatia
  • S Čolak Cromaris d.d., Ilirska cesta bb, 23232 Nin, Croatia
  • J Aladrović Department of Physiology and Radiobiology, Faculty of Veterinary Medicine University of Zagreb, Heinzelova 55, 10000 Zagreb, Croatia
  • I Strunjak-Perović Laboratory for Ichthyopathology-Biological Materials, Rudjer Bošković Institute, Bijenička cesta 54, 10000 Zagreb, Croatia
  • N Topić Popović Laboratory for Ichthyopathology-Biological Materials, Rudjer Bošković Institute, Bijenička cesta 54, 10000 Zagreb, Croatia
  • L Vranković Department of Physiology and Radiobiology, Faculty of Veterinary Medicine University of Zagreb, Heinzelova 55, 10000 Zagreb, Croatia
  • R Čož-Rakovac Laboratory for Ichthyopathology-Biological Materials, Rudjer Bošković Institute, Bijenička cesta 54, 10000 Zagreb, Croatia

DOI:

https://doi.org/10.25431/1824-307X/isj.v14i1.379-387

Keywords:

crustaceans, hemolymph, antioxidant enzymes, lipids

Abstract

The aim of this study was to determine interspecies and gender differences in the lipid metabolism and hemolymph oxidation stability in intermoult period of the European spider crab (M. squinado), the warty crab (E. verrucosa) and the European spiny lobster (P. elephas). In hemolymph samples activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and paraoxonase 1 (PON 1), as well as hemolymph cholesterol, triacylglycerol and non-esterified fatty acid (NEFA) concentrations were determined. The hemolymph of M. squinado showed the highest levels of SOD activity, cholesterol and triacylglycerol and the lowest levels of PON 1 activity compared to values determined in the hemolymph of E. verrucosa and P. elephas. Gender differences were determined only in M. squinado samples. Males had significantly higher activity of PON 1 and lower NEFA values compared to females. M. squinado hemolymph lipid metabolism and oxidation stability in intermoult period could be attributed to their biological, metabolic characteristics and environmental conditions in which these species live. 

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Published

2017-09-25

Issue

Vol. 14 No. 1 (2017)

Section

Research Reports