Oxidative stability of the hemolymph in different crustacean species
Keywords:crustaceans, hemolymph, antioxidant enzymes, lipids
The aim of this study was to determine interspecies and gender differences in the lipid metabolism and hemolymph oxidation stability in intermoult period of the European spider crab (M. squinado), the warty crab (E. verrucosa) and the European spiny lobster (P. elephas). In hemolymph samples activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and paraoxonase 1 (PON 1), as well as hemolymph cholesterol, triacylglycerol and non-esterified fatty acid (NEFA) concentrations were determined. The hemolymph of M. squinado showed the highest levels of SOD activity, cholesterol and triacylglycerol and the lowest levels of PON 1 activity compared to values determined in the hemolymph of E. verrucosa and P. elephas. Gender differences were determined only in M. squinado samples. Males had significantly higher activity of PON 1 and lower NEFA values compared to females. M. squinado hemolymph lipid metabolism and oxidation stability in intermoult period could be attributed to their biological, metabolic characteristics and environmental conditions in which these species live.