Characterization of DDX21 gene and the association of its single nucleotide polymorphisms with susceptibility/resistance of Magallana gigas to Halomonas sp. 7T

Authors

  • Q Peng Liaoning Key Laboratory of Marine Animal Immunology & Disease Control, Dalian Ocean University, Dalian, 116023, China ; Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, 116023, China ; Dalian Key Laboratory of Aquatic Animal Disease Prevention and Control, Dalian Ocean University, Dalian, 116023, China
  • T Liu Liaoning Key Laboratory of Marine Animal Immunology & Disease Control, Dalian Ocean University, Dalian, 116023, China ; Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, 116023, China ; Dalian Key Laboratory of Aquatic Animal Disease Prevention and Control, Dalian Ocean University, Dalian, 116023, China
  • C Yang Liaoning Key Laboratory of Marine Animal Immunology & Disease Control, Dalian Ocean University, Dalian, 116023, China ; Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, 116023, China ; Dalian Key Laboratory of Aquatic Animal Disease Prevention and Control, Dalian Ocean University, Dalian, 116023, China
  • N Kong Liaoning Key Laboratory of Marine Animal Immunology & Disease Control, Dalian Ocean University, Dalian, 116023, China ; Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, 116023, China ; Dalian Key Laboratory of Aquatic Animal Disease Prevention and Control, Dalian Ocean University, Dalian, 116023, China
  • B Wang Liaoning Key Laboratory of Marine Animal Immunology & Disease Control, Dalian Ocean University, Dalian, 116023, China ; Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, 116023, China ; Dalian Key Laboratory of Aquatic Animal Disease Prevention and Control, Dalian Ocean University, Dalian, 116023, China
  • Y Wang School of Fisheries and Life Science, Dalian Ocean University, Dalian, 116023, China
  • L Wang Liaoning Key Laboratory of Marine Animal Immunology & Disease Control, Dalian Ocean University, Dalian, 116023, China ; Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, 116023, China ; Dalian Key Laboratory of Aquatic Animal Disease Prevention and Control, Dalian Ocean University, Dalian, 116023, China
  • L Song Liaoning Key Laboratory of Marine Animal Immunology & Disease Control, Dalian Ocean University, Dalian, 116023, China ; Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, 116023, China ; Dalian Key Laboratory of Aquatic Animal Disease Prevention and Control, Dalian Ocean University, Dalian, 116023, China

DOI:

https://doi.org/10.25431/1824-307X/isj.v22i1.91-106

Keywords:

Magallana gigas, DDX21, promoter, single nucleotide polymorphism, resistance to Halomonas sp. 7T

Abstract

DEAD-box RNA helicase 21 (DDX21) is a widely expressed protein in cells, involved in almost all RNA-related cellular processes, and plays a significant role in disease prevention and defense within organisms. This study characterized a DDX21 in Pacific oyster Magallana gigas (MgDDX21), and investigated its association of single nucleotide polymorphisms with susceptibility/resistance of oyster to Halomonas sp. 7T. MgDDX21 contains three conserved domains (DEADc, HELICc, and GUCT), and shares highly conserved key functional sites with other organisms. High-temperature and Lipopolysaccharide (LPS) treatment both significantly upregulated the mRNA expression level of MgDDX21 (P < 0.05). The promoter sequence of MgDDX21 gene from the bacterial-resistant population and the common population was cloned, and the polymorphisms within this region were investigated by sequencing to analyze their association with bacterial resistance. A total of 25 single nucleotide polymorphism (SNP) sites were identified, and 21 SNPs and one haplotype TACACTAACT were significantly associated with the bacterial resistance trait of Pacific oyster, which could be used as potential markers for oyster selection breeding with higher bacterial resistance. These results lay the foundation for further investigation into the role of DDX21 in the response of Pacific oyster to bacterial resistance and provide candidate molecular markers for breeding new varieties of oysters with excellent disease resistance traits.

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Published

2025-10-22

Issue

Vol. 22 No. 1 (2025)

Section

Research Reports